Sleep patern disturbance makes the body unhealthy, as a result the autonomic nervous system imbalance. It decrease the value of Heart Rate Variability (HRV), then the risk of morbidity and mortality increases. Objective: This research purpose is to know the relationship between sleep pattern disturbances with HRV in 8 nursing student Universitas Muhammadiyah Yogyakarta. Methodology: This research was non experimental study with correlational design and cross sectional approach. This research samples was 31 male student which choosen by purposive sampling techiques. Sleep pattern disturbances and HRV was measured by questionnare and Electrocardiogram (ECG). The data was analyzed by Sperman Rho. Result: There are no correlation Sleep pattern disturbance and HRV with p = 0,210 (p > 0, 05). Consclusion: There are no correlation between sleep patterns disturbances with HRV.