Baby milk bottle sterilization based microcontroller is a tool that serves to kill micro-organisms including bacterial spores. Kesterilan tools is absolutely essential in particular tools that the direct contact with humans as well as on baby milk bottle, a lot of bacteria or germs that must be sterilized so as not to make of diseases which can interfere with the growth of the baby. On the module principle works by utilizing the heat from the heater glass as sterilization baby milk bottle with a time of 5 minutes and a temperature of 100oC which is controlled by the mikrokonroller and Atmega8535 LM35 temperature as a reader. Based on the results of the temperature measurement error of results obtained 0.08% and time measurement data obtained results an error of 0.02%. If the average error on every data capture of measurement value percentage of 5% then < modules can be feasible, with shows the level of confidence in the research of over 99% and a level of probability (the chance of errors) less than 1%. And based on the results of a test lab that has done as much as 10 times the testing in the laboratory of Microbiology, Faculty of medicine and Health Sciences University of Muhammadiyah in Yogyakarta can be concluded that, in the circumstances that have not been sterilized bottles there are bacteria on average 128.3 colonies and sterilized after on average 0.7 colony. Thus it can be concluded that the author of the module create effective and able to kill bacteria in the milk bottle baby.