Sport is a way to reach a healthy body and fitness. Sport depends on muscle metabolism consist of aerobic sport, like gymnastic, and anaerobic sport, like bodybuilding. Fit lifestyle through bodybuilding or aerobic gymnastic will cause some substantial changes inside the body. The substantial changes can be used as a mark of body metabolism alteration, mainly the function of metabolism organ, like liver and renal. Albumin is main plasma protein synthesized by liver and has big role to rule plasma colloid osmotic pressure and as protein transport. This research is needed to know the difference of albumin level between bodybuilding enthusiasts and aerobic gymnastic enthusiasts. This is a quantitative research with non-experimental design. This research used analytic-observational with cross sectional survey approach. This research is done by collected blood samples of bodybuilding enthusiasts at Adonis Fitness Center and aerobic gymnastic enthusiasts at Sanggar Senam Adinda Yogyakarta on August 2016. Albumin levels are examined from the blood sample in Balai Laboratorium Yogyakarta. Total samples is 40 samples consisted of 20 bodybuilding enthusiasts and 20 aerobic gymnastic enthusiasts. Data is analyzed with Independent T-Test. Analyzed data with independent t-test showed p value= 0,010. Albumin level of bodybuilding enthusiasts has average value 4,776 g/dL, it is higher than albumin level of aerobic gymnastic enthusiasts which has average value 4,590 g/dL. The difference of albumin level between bodybuilding enthusiasts and aerobic gymnastic enthusiasts is 0,186. This research concluded that there is difference of albumin level between bodybuilding enthusiasts and aerobic gymnastic enthusiasts.