Background: Healthy lifestyle can be done with positive activities such as sports, physical activity and doesn’t apply sedentary lifestyle. Sedentary behavior contributes to all-cause mortality, one of which is associated with cardiovascular disease.Cardiovascular health can be seen through the Heart Rate Variability ( HRV ). Objective: This research purpose is to know the difference HRV between active physical activity groups and sedentary groups in Nursing Science Program students of VIII semester Muhammadiyah University of Yogyakarta. Methodology: This research is a quantitative research. To know the difference HRV between active physical activity groups and sedentary groups which is measured with electrocardiogram ( ECG ). Respondents consisted of 30 people of which 15 respondents were students with an active physical activity and 15 were students with sedentary activities. Result: There is no difference Heart Rate Variability ( HRV ) in students with active physical activity and sedentary. It can be seen from Mean -Whitney test , the obtained value of p = 0073. Conclusion: There is no difference Heart Rate Variability ( HRV ) in active physical activity students and sedentary students.