The incidence in prevalence of diabetes mellitus type 2 increased in the whole world. International Diabetes Federation (IDF) predicts that Indonesia has increasing of amount of diabetic patient 10 million in 2015 to be 16,2 million in 2040. The adherence of patients with treatment of chronic disease like diabetes melitus mostly was poor. Telemedicine contains giving information and education such as multimedia messaging specifically animation video using messages application internet-based on smartphone. The research has purpose to know the influence of Telemedicine (messages application internet-based) on the adherence of medication in patient with type 2 diabetes melitus. Methods: The design of this research was a Quasi-Experiment pretest and posttest with control group design. The subjects were 56 patient with type 2 diabetes melitus that routinely check up in PKU Muhammadiyah Yogyakarta Hospital, each consisting of 28 patients in intervention group and the control group. Interventions was administered for 8 times and twice per week for one month. The adherence of medication score was evaluated with Morisky Medication Adherence Scales (MMAS‐8). The data was analyzed by Wilcoxon test and Mann-Whitney test. Results: The result of Wilcoxon Signed Rank Test obtained significance value 0,539 for intervention group and 0,071 for control group. The results of 2 samples Mann-Whitney test calculation of mean rank values for intervention group is 31,95 and 25,05 for control group with p value 0,098. Conclusions: Telemedicine (messages application internet-based) can improve the adherence of medication in intervention group patient with type 2 diabetes melitus but not statistically significant.