| dc.contributor.author | PATRAMURTI, CHRISTINE | |
| dc.date.accessioned | 2017-07-24T05:50:17Z | |
| dc.date.available | 2017-07-24T05:50:17Z | |
| dc.date.issued | 2012-12-05 | |
| dc.identifier.issn | 978-602-9420-29-6 | |
| dc.identifier.uri | http://repository.umy.ac.id/handle/123456789/11765 | |
| dc.description | A reversed-phase liquid chromatography with ultraviolet detection is described fr the determination of cotinine, 3-hydroxycotinine and also 3-hydroxycotinine/cotinine ratio. A mobile phase consisted of a mixture ammonium acetate:methanol (50:50). Using a CS-type column endcapping, a good separation was achieved in 8 mm. The wavelength of tJV detector was set at 260 nm. With acetanilide as internal standard, human urine samples were cleaned up by liquid-liquid extraction. The regression equations obtained from both the standard were linear between 1.0 - 5.0 ig/mL for 3-hydroxycotinine and between 2.0-10.0 .ig/mL (r> 0.999). This method has been successfully applied to the determination of urinary 3-hydroxycotinine and cotinine content and also for detennining 3-hydroxycotinine/cotinine ratio in several Javanese smokers from Indonesia. | en_US |
| dc.description.abstract | A reversed-phase liquid chromatography with ultraviolet detection is described fr the determination of cotinine, 3-hydroxycotinine and also 3-hydroxycotinine/cotinine ratio. A mobile phase consisted of a mixture ammonium acetate:methanol (50:50). Using a CS-type column endcapping, a good separation was achieved in 8 mm. The wavelength of tJV detector was set at 260 nm. With acetanilide as internal standard, human urine samples were cleaned up by liquid-liquid extraction. The regression equations obtained from both the standard were linear between 1.0 - 5.0 ig/mL for 3-hydroxycotinine and between 2.0-10.0 .ig/mL (r> 0.999). This method has been successfully applied to the determination of urinary 3-hydroxycotinine and cotinine content and also for detennining 3-hydroxycotinine/cotinine ratio in several Javanese smokers from Indonesia. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Muhammadiyah Tobacco Control Center | en_US |
| dc.subject | 3-hydroxycotinine/cotinine ratio, HPLC, human urine | en_US |
| dc.title | ANALYSIS OF RATIO 3-HYDROXYCOTININE: COTININE: PHENOTYPING STUDY OF CYTOCHROME P450 2A6 ON INDONESIAN SMOKER | en_US |
| dc.type | Book | en_US |
