Off-label drugs are drugs that are used not in accordance with official drug information and do not have permission to use outside the provisions given. As for drugs that are categorized off-label if the drugs used not in accordance with the indication listed on the leaflet. There are many reports related with incidences of off-label drug use in children. This study aims to determine the prevalence of off-label drug use in pediatrics departemen of RS PKU Muhammadiyah Bantul. This research used descriptive observational design with cross-sectional method. Off-label prescription drug data collection is done retrospectively. The sampling technique used is Simple Random Sampling from January to October 2016. Samples are then analyzed based on the list of drugs listed in the National Drug Information Center (PIO Nas) classified under Anatomical Therapeutical Chemical (ATC). Based on this study obtained a total sample of 403 receipt of patients with 1,085 types of drugs. From the analysis, there were 4 drugs prescription (0.37%) consisting of three types of drugs included in the off-label drug indication: valproic acid (2 presscription), chloralhydrate (1 prescription) and ondansetron (1 prescription).