The use of medicine was considered as rational step if the patients received a medicine which was suitable to the requirements, appropriate dosage, suitable duration, and cost to pay the medicine. The use of rational medicine was available in WHO’S indicator in 1993. WHO indicator in 1993 became a standard to use medicine in medic in which the indicator was separated to be prescribing indicator, treatment indicator, and medical facility indicator. This research aimed to reveal the use of medicine in RSUD Panembahan Senopati Bantul with prescribing indicator based on WHO 1993, and to reveal the level of patient satisfaction in pharmacy installation RSUD Panembahan Senopati Bantul. This research was descriptive research non-experiment. The data was taken by using retrospective which meant the prescription of patient in September 2016- November 2016 was collected, and to the researcher also distributed a questioner to the patients. The method of this research to take the sample was random sampling system. To analysis the data, this research took the percentage every prescription which was compared with the standard of WHO 2013. To analyze the questioners with 21 questions were checked by using 5 aspects of service quality. This research showed that the similarity of medicine with prescription indicator based on WHO 1993 was the average of medicine each prescription which was as much as 2,63 items. The percentage of generic medicine was 79,58%, percentage of antibiotic prescription was 30,33%, percentage of injection was 1,33%, percentage of formulation was 92,20%, and the level of patient satisfaction was 3% which revealed that they were very satisfied, 24% satisfied, 58% satisfied enough, 14% less satisfied, 1% not satisfied. According to the research, the indicator of prescription was not enough to fulfill the standard of WHO 1993 yet, and there were some patients who still felt not satisfied given by the pharmacy installation in RSUD Panembahan Senopati Bantul based on 5 aspects of service quality, the largest percentage stated quite satisfied with the value of service quality aspects sorted from the highest are tangible, reliability, responsiveness, assurance and empathy.