Background: Porphyromonas gingivalis is a common pathogenic bacterium corresponding to the occurrence of periodontal disease. In order to treat periodontal diseases, several synthetic medicaments can be applied as a supportive periodontal treatment. Beside those synthetic medicaments, as an alternative non-synthetic medicament that can be applied is natural biological compound-related medicament harboring antibacterial potency. One of the candidate is ethanol extract propolis (EEP). Purpose: This study aims to assess the growth of the Porphyromonas gingivalis due to the addition EEP from Apis Trigona sp. Methods: This research was conducted by experimental laboratory in vitro. Liquid dilution method was used using Tryptose Phosphate Broth media while the measurement of turbidity was performed by using Spectrophotometer UV-vis. Extracts ethanol of propolis from Apis Trigona sp were tested on the bacteria Porphyromonas gingivalis into some concentrations: 0,8%; 0,4%; 0,2%; 0,1;, and 0,05% based on the weight/volume (w/v). Results: All tested concentrations can inhibit the growth rate of Porphyromonas gingivalis bacteria. The most effective concentration against bacterial growth rate was 0.4% (p <0.05). Conclusion: The EEP from Apis Trigona bees effectively inhibit the growth of Porphyromonas gingivalis bacteria.