| dc.contributor.advisor | MAHANANI, ERLINA SIH | |
| dc.contributor.author | KHAIRUNNISA, NADYA | |
| dc.date.accessioned | 2018-02-10T02:35:06Z | |
| dc.date.available | 2018-02-10T02:35:06Z | |
| dc.date.issued | 2017-05-09 | |
| dc.identifier.uri | http://repository.umy.ac.id/handle/123456789/17654 | |
| dc.description | Degradasi perancah merupakan salah satu faktor penting pada rekayasa
jaringan untuk proses pembentukan jaringan baru. Platelet-rich plasma (PRP)
merupakan komponen darah kaya growth factor. Proses degradasi perancah
hidrogel yang diinkorporasikan dengan PRP terjadi bersamaan dengan perilisan
growth factor yang di hasilkan oleh PRP.
Penelitian ini bertujuan untuk mengetahui pengaruh inkorporasi PRP
terhadap proses degradasi perancah koral buatan CaCO3. Penelitian ini
menggunakan desain eksperimental laboratorium in vitro. Platelet-rich plasma
dibuat berdasar metode Tabata yaitu dengan metode double spinning. Darah
diambil dari tikus wistar melalui vena lateral ekor. Perancah dibagi menjadi dua
kelompok yaitu perancah inkorporasi PRP (n=3) dan perancah non-inkorporasi
PRP sebagai control (n=3). Perancah diinkorporasikan dengan PRP dengen
mencelupkan perancah kedalam 75l PRP selama 15menit. Perancah inkorporasi
dan non-inkoporporasi PRP di inkubasi selama 1jam. Setiap interval waktu yaitu
pada jam ke 1, 3, 6, 24, 48 dan 96 larutan PBS diganti yang baru dengan volum
yang sama. Setelah jam ke-96 larutan diganti dengan HCl 1N sebagai larutan
akselerasi kemudian diinkubasi pada interval waktu yang sama seperti PBS.
Analis data dengan Independent sample t Test. Hasil analis data
menunjukkan tidak terdapat perbedaan bermakna antara perancah yang
diinkoporasi PRP dengan perancah tanpa inkorporasi. Namun berdasarkan grafik
rata-rata persentase degradasi terlihat pergerakan kurva perancah tanpa
inkorporasi PRP lebih pendek dibandingkan dengan perancah inkorporasi PRP.
Kesimpulan dari penelitian ini terdapat pengaruh PRP yang diinkoporasi dengan
perancah koral buatan CaCO3 terhadap profil degradasi. | en_US |
| dc.description.abstract | Degradation of scaffold is an important factor in the tissue engineering to
form a newly tissue. Platelet-rich plasma (PRP) is one of bloods component which
nourished by growth factor. Hydrogel scaffold degradation process which
incorporated with PRP at the same time also created growth factor that is made
by PRP. Aimed of this study is to develop the respons of PRP to the degradation
profile on CaCO3 scaffold.
Platelet-rich plasma preparation was use the method from Tabata by
double-spinning method. Blood was taken from lateral vein of wistar rats (rattus
norvegicus). Six synthetic coral scaffolds were divided into two groups. First is
PRP incorporation group (n=3) and the second is synthetic coral scaffolds
without PRP as the control (n=3). Synthetic coral scaffolds dipped into 75l of
PRP for 15minutes. Incorporated and non-incorporated PRP on both sides were
placed in 1 ml of PBS then incubated for 1 hours at 370C. At different time
intervals 1, 3, 6, 24, 48 and 96 hours the solution supernatant was removed and
replaced with the same volume of fresh PBS solution. After 96 hours the solutions
were changed with HCl 1N for accelerate solutions the incubated at same time
intervals like PBS.
The Independent T test showed no significant different on both
incorporated or non-incorporated PRP. Meanwhile, based on the average
graphic percentage a degradation showed movement on scaffolds curves without
non-incorporated PRP was lesser than incorporated PRP. The conclusion, this
study explains the influences of incorporated PRP with CaCO3 scaffolds on
degradation profile. | en_US |
| dc.publisher | FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS MUHAMMADIYAH YOGYAKARTA | en_US |
| dc.subject | Tissue Engineering, artificial scaffolds CaCO3, Platelet-rich plasma, Degradation. Rekayasa Jaringan, Perancah koral buatan CaCO3, Platelet-rich plasma, Degradasi. | en_US |
| dc.title | PENGARUH INKORPORASI PLATELET-RICH PLASMA PRP PADA PERANCAH KORAL BUATAN CaCO3 TERHADAP PROFIL DEGRADASI | en_US |
| dc.type | Thesis
SKR
FKIK
398 | en_US |
