Irrigation is an important step in the treatment diabetic foot ulcers particularly for avoiding infection spread. In the previous study, the irrigation using artrhipi was effective for diabetic foot ulcers, it based on wound recovery score. This study aims to investigate the effectiveness of artrihpi based on the load of the bacteria. Randomized controlled trial (RCT) with a parallel design and double-blind was used as the method. Twenty-two respondents with diabetic foot ulcers in hospital participated in this study. After randomizing step, the respondents were treated by using artrihpi (with pressure 10-15psi) or spuit 12 cc with needle number 22 (with pressure 13 psi). Before and after the wound care, the wound had to swab for analyzing of an amount the bacterial.Wilcoxon and Mann-Whitney were used for analyzing the bacterial load. The result of this study shows there was statistical difference between the load of bacteria pre and post the irrigation in both intervention group (p value: 0,041) and control group (p-value: 0,006). There was no statistical different between intervention and control group (p-value: 0,25). The differences amount of bacterial before and after irrigation in the intervention group was more than the control group. This study recommends the wound irrigation using artrihpi as one of nursing intervention toward diabetic foot ulcers.