Background : Number of infection in hospital is still high. This infection is called Health-care Associated Infection (HAIs) in which the incidence of HAIs causes 1.4 million people die every day around the world. Medical or non-medical workers, not exception to outsourcing workers, in the hospital are risk to transmission the infection. The transmission of this infection the most is by the hand through the hands. According to WHO, hand hygiene which consists of hand wash and hand rub is one of the activities that can reduce the spread transmission of the infection, so that it can improve the hand hygiene and stop the spread of infection. Which are there are many factors that affect the hand hygiene, including the level of education, knowledge and abilities. Methods : the type of research was observational research with cross sectional approach, in PKU Muhammadiyah Gamping Hospital with 57 outsourcing workers as respondents by total sampling as the sampling technique. Result : The result showed there is no significant correlation between educational level with hand hygiene ability (p = 0,091). However, there is significant correlation between knowledge level with hand hygiene ability (p = 0,001).