Background: Allergy is specific reaction of the immune system to stimuli of a material that other people are usually not harmful. This material is called allergen. Allergic reaction is caused by an immune response that recognize the allergen as a foreign object and immediately formed a large number of antibody called immunoglobulin E. IgE can be detected on the skin with skin prick test. Skin prick test is based on the reaction between the antigen and sensitized mast cells in the skin that produce a response by the form of wheals and reddish. Allergic diseases increase globally up to 30-40% of the population. There are one or more allergic conditions and affect the children. When the children is growing up, the severity of allergic diseases will increase. Thus, will interfere the quality of children life. Methods: Cross-sectional study was conducted on 58 elimentary students in rural and urban areas, aged 10-12 years old. Subjects was examined with skin prick test by using 10 allergens and 2 controls. Subjects also filled the questionnaire for evaluated the risk factor of atopic. The aim of this study is to determine the comparison of skin prick allergy test in rural and urban children. Result: The positive skin prick test is higher in urban than rural areas (58.6%: 34.5%). The highest positive allergen in rural and urban areas is house dust (36.2%) and the highest negative allergens are shrimp, fish, chicken egg whites, and tea (0%). There is no difference of skin prick test result in rural and urban areas (p=0.065). Those results are affected by self-hygiene, good sanitations, and advanced transportations in rural areas. Conclusion: There is no difference of skin prick test result in rural and urban areas. The highest positive allergen in rural and urban areas is house dust.