| dc.contributor.advisor | SIH MAHANANI, ERLINA | |
| dc.contributor.author | NURLAELI, MAULIDA | |
| dc.date.accessioned | 2018-10-09T07:00:14Z | |
| dc.date.available | 2018-10-09T07:00:14Z | |
| dc.date.issued | 2018-07-17 | |
| dc.identifier.uri | http://repository.umy.ac.id/handle/123456789/21960 | |
| dc.description | Latar belakang: Platelet-rich Plasma (PRP) didefinisikan sebagai trombosit
terpekatkan yang mengandung banyak faktor pertumbuhan. PRP memiliki peran
penting dalam proses penyembuhan tulang. Platelet-rich Fibrin (PRF) merupakan
pengembangan konsentrat platelet yang tidak memanfaatkan faktor anti koagulan.
PRF dissebut juga sebagai leukosit-PRF karena memiliki sifat antisipasi dalam
meregenerasi jaringan dan penyembuhan luka. PRP dan PRF dapat di
inkorporasikan dengan perancah dalam rekayasa jaringan tulang. Bahan material
perancah memiliki sifat tidak mudah larut atau degradasi agar proses pelepassan
faktor pertumbuhan dapat sesuai dengan proses pembentukan jaringan tulang.
Tujuan Penelitian: Penelitian ini bertujuan untuk mengetahui perbedaan profil
degradasi pada perancah yang diinkorporasi dengan PRP, PRF dan tanpa
inkorporasi.
Metode Penelitian: Desain penelitian ini adalah penelitian klinis laboratoris
menggunakan pre test post test design. Subjek penelitian yaitu perancah koral
buatan. PRP di buat dengan menggunakan metode Matsui-Tabata. PRF di buat
dengan menggunakan metode Choukroun dkk. Darah yang digunakan merupakan
darah yang berasal dari manusia. Sebanyak 9 perancah dibagi menjadi 3
kelompok yaitu perancah dengan inkorporasi PRP, inkorporai PRF dan tanpa
inkorporasi. Subjek penelitian direndam dalam PBS dan diinkubasi pada suhu 37o.
pengukuran profil degradasi dilakukan pada periode waktu 1, 3, 6, 24, 48, 72, dan
96 jam. Kemudian mengganti larutan PBS dengan HCl 1N dan diinkubasi pada
suhu 37o. pengukuran kembali profil degradasi pada periode waktu 1, 3, 6, 24, 48,
72, 96 jam hingga perancah habis terdegradasi.
Hasil Penelitian: Data hasil penelitian dianalisis menggunakan uji One Way
Anova dan dilanjutkan dengan uji Post Hoc dengan Tukey. Hasil uji One Way
Anova menunjukkan terdapat perbedaan bermakna (p<0.05) pada perendaman
PBS periode waktu 1, 3, 6, 24, 48, 72, 96 jam dan perendaman HCl periode waktu
1 jam.
Kesimpulan: berdasarkan hasil penelitian ini terdapat perbedaan degradasi antara
perancah dengan inkorporasi PRP, PRF dan tanpa inkorporasi. Penambahan PRP
dapat memperkuat struktur dari perancah dengan pembentukan fibrin network
sehingga dapat memperlambat proses degradasi. | en_US |
| dc.description.abstract | Background: Platelet-rich Plasma (PRP) is defined as concentrated platelet that
consists of a great amount of growth factors. PRP has important role in bone
recovery process. Platelet-rich Fibrin is the development of concentrated platelet
that does not use anti coagulant. PRF is also called as leukocyte-PRF since it has
anticipation character in tissue regeneration and wound recovery. PRP and PRF
can be incorporated using scaffolding in engineering bone tissue. Scaffolding
materials are insoluble or degradation in order that the release of growth factors in
accord with bone tissue development process.
Research objective: The research aimed at learning the difference of degradation
profile in the scaffolding incorporated with PRP, PRF, and without incorporation.
Research Method: The research design is clinical laboratory research using pre
and post test design. The subject of the research is artificial coral scaffolding. PRP
was made using Matsui-Tabata method. PRF was made using a method by
Choukron et all. The blood used was human blood. 9 scaffoldings were divided
into 3 groups; scaffolding with PRP incorporation, PRF incorporation, and
without incorporation. The artificial coral scaffolding was soaked in PBS and was
incubated with the temperature of 37o. Degradation profile was measured after 1,
3, 6, 24, 48, 72, and 96 hours. PBS was then replaced with HC1 1N and was
incubated with the temperature of 37o . Degradation profile was measured again
after 1, 3, 6, 24, 48, 72, 96 hours until the scaffolding was degraded until it ran
out.
Research Result: The data of the research were analyzed using One Way Anova
and continued with Post Hoc test using Tukey. The result of One Way Anova
indicated that there was significant difference (p<0.05) in the soaking period of 1,
3, 6, 24, 48, 72, 96 hours and 1 hour period of HC1 soaking.
Conclusion: Based on the research, it is concluded that there was degradation
difference between scaffolding with PRP incorporation, PRF incorporation, and
without incorporation. PRP addition could strengthen the scaffolding structure
with the development of fibrin network so that it could slow down the degradation
process. | en_US |
| dc.publisher | FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS MUHAMMADIYAH YOGYAKARTA | en_US |
| dc.subject | Degradation, Platelet-rich Plasma, Platelet-rich Fibrin, Artificial Coral Scaffolding | en_US |
| dc.title | PERBEDAAN PENAMBAHAN PLATELET RICH PLASMA DAN PLATELET RICH FIBRIN TERHADAP PROFIL DEGRADASI PADA PERANCAH REGENERASI TULANG | en_US |
| dc.type | Thesis
SKR
FKIK
236 | en_US |
