Background: To improve public health, hospital has very vital role as health facility. Many studies show undesirable incidences and complications of Healthcare Associated Infections (HAIs) in the past decades. Based on the 2012 accreditation standard of Hospital Accreditation Committee (KARS), PPI 7.1 standard is hospital reduces risk of infection by ensuring adequate equipment cleaner and sterilization, as well as appropriate laundry and linen management. The objective of this research is to analyze linen management on the infection prevention and control of PKU Muhammadiyah Gamping Hospital. Subject and Method: This was a qualitative explorative study and the research design was case study. The informants in this study was all employees of the laundry or linen unit of PKU Muhammadiyah Hospital, Gamping (11 people), and 3 members of the board of directors, i.e. linen supervisor, infection and prevention control unit supervisor and household manager. This research was conducted in July 2018 which is located in PKU Muhammadiyah Gamping Hospital. Data collection instrumen are questionnaire, observation checklist, and interview. Result: Linen staff has good perspective on linen laundry management, as 6 participants (55%). In the stage of collecting linen and washing, 20% staff didn’t comply with complete Personal Protective Equipment (PPE). The perspective of the management on linen laundry management was good. The obstacles of the linen staffs in performing the standard operating procedure of linen management of PKU Muhammadiyah Gamping Hospital, were using clean and closed trolley facility and high workload. Conclusion: The level of compliance of linen staffs on the standard operating procedure of linen management wasn’t optimal. More staffs were required to reduce the staffs workloads and the facilities should be improved. Keywords: Linen management, Infection Prevention and Control (PPI), PKU Muhammadiyah Gamping Hospital