Background: The national program Expanding Maternal And Neonatal Survival (EMAS) is motivated by the large proportion of pregnancy at ages which are too young and too old. According to 2010 civil census, over 30% deaths happen in women younger than 20 and older than 35. The implementation should be supported by good health worker performance so that the reduction of mortality rate can achieve the targets. The purpose of this study was to determine the difference of health worker performance in EMAS program hospital and Non-EMAS program hospital. Method: The research type was quantitative with difference test analysis. The research samples came from 2 hospitals, consisting of 30 health workers from EMAS program hospital and 30 health workers from Non-EMAS program hospital. The data was collected using questionnaire and the data analysis used Mann Whitney test. Performance included ability, attitude, motivation, leadership and facility and infrastructure. Result: The result of the difference test on 5 performance factors showed that 3 factors, i.e. ability, attitude and facility and infrastructure in EMAS program hospital had better scores than Non-EMAS program hospital. However, 2 other factors didn’t show any difference, i.e. motivation and leadership. There was significant difference in attitude. Conclusion: The performance of hospital with EMAS program was different in terms of attitude from hospital without EMAS program. Keywords: Performance, EMAS Program, Health Worker Performance