Aloe Vera is a natural polymer that is easily available and is widely developed in this modern era, Aloe Vera has many benefits, especially in the biomedical field. One of them was applied as a wound dressing membrane. This study aims to fabricate natural PVA/Aloe Vera nanofiber membranes using electrospinning method by characterizing morphology and its tensile properties. Fabrication of natural nanofiber membranes made from Aloe Vera polymer polyvinyl alcohol (PVA) blend is done first by smoothing the natural Aloe Vera gel which is then carried out the screening process. Spinning solution is made using a blending method with a ratio (10 distilled water (g): PVA 1 (g): natural concentration of Aloe Vera 0, 10, 20 and 30%) w/w. Making nanofiber membrane with electrospinning method at a voltage of 15 kV, tip distance to collector (TCD) 16 cm, and needle diameter 0.7 mm. Characterization of the physical properties of nanofiber fiber membranes was carried out using a scanning electron microscope (SEM) while the mechanical properties (tensile test) were tested using universal testing machine tensile testing machine (Zwick 0.5 Germany, ASTM D 882). The results of the analysis of the physical properties of nanofiber membranes using SEM showed that more PVA / AV concentrations would increase the diameter size of nanofiber, at a concentration of 0% 136 nm fiber diameter while at a concentration of 30% to 223 nm. Furthermore, the results of the analysis of the mechanical properties of nanofiber membranes showed that with the increasing concentration of natural Aloe Vera it was able to increase the value of tensile strength (2,598-3,585 MPa), strain (105,864-158,204%) and modulus of elasticity (16,305-17,721 MPa). The results of this study have the potential to be used as wound dressing.