Background :Smoking is activities that have bad effects for health. Cigarette is a bunch of aditive substance that dangerous for our body. The dopamine in the brain of smoker will beincrase and make addiction on smoking, in the other side smoking can affect the level concentration of person Objective :The result of this research is to know level concentration on smoker and non smoker in Agriculture Faculty University Muhammadiyah Yogyakarta. Research Method :This research is Non Eksperimental with descriptive comparative design. The number in this sample are 80 sample with Total Sampling technique. The data analyze using Mann Whitney Test. The Result :The result in this research there are have a differences of level concentration in smoker and non smoker p=0,009 (p<0,05) The concusion : The level of concentration of non smoker more higher than the level concentration ofsmoker