Urinary Tract Infection (UTI) is a general term thst used to express the invasion of microorganisms in the urinary tract. UTI disease in the United States caused more than 7 million doctor visit every year and about 15% of antibiotics in the United States were used for UTI (Grabe M et al., 2015). While the prevalence of UTI in Indonesia was 90-100 per 100,000 population and 222 million people in Indonesia have experienced UTI (MOH R1, 2014). The purpose of this study was to determine the profile of antibiotic in UTI disease and to determine the suitability of antibiotic therapy that given at PKU Muhammadiyah Gamping Hospital with UTI therapy guidelines based on the exact drug, right dose, right patient, right indication. This research was a descriptive observational study used cross sectional design with retrospective data retrieval and purposive sampling technique. Data retrieval used secondary data in the form of medical record records. Then the description and accuracy data with the guidelines were analyzed in the form of percentages. Data took in the form of the used of drugs for UTI patients from January to December 2016. In this study 109 samples were obtained. There were 2 types of antibiotic use, namely single and combination. Distribution of single antibiotic use was ceftriaxone 41.7%, ciprofloxacin 15.7%, cefixime 15.7%, levofloxacin 12.0% and cefotaxime 14.8%. The use of combination antibiotics were ceftriaxone - levofloxacin 20%, ceftriaxone - ciprofloxacin 30%, cefotaxime - levofloxacin 20%, ceftriaxone - cefixime 30%. Data suitability obtained with the Urinary Tract Infections guideline was the exact indication 100%, the exact drug 29.4%, the accuracy of the patient 90.70%. For the right dose compared to the formulary PKU Muhammadiyah Hospital Gamping 2016 was 100%.