Background: Healthy conditions and illness can be determined from his attitude. The attitude of healthy life is influenced by the level of intelligence. Intellectual intelligence is only 20% acting in attitude, the rest there is another important role of intelligence is the spiritual intelligence. Objective: to know the effect of spiritual intelligence training on the establishment of healthy life attitude of factory labor in Yogyakarta. Method: This research uses experimental design with pre-post test control design method and collecting data by purposive sampling. Implementation of training at glove factory in Yogyakarta. The subjects were divided into 2 groups: control and treatment. The control group consisted of 10 respondents of factory labor who were not trained. The treatment group consisted of 10 respondents of factory labor who were given spiritual intelligence training for 5 days. Treatment groups were given 3 spiritual material sessions each day. Both groups before and after the training performed measurements of spiritual intelligence as well as a healthy lifestyle with questionnaire tools that have validated product moment and reliably tested Alpha Cronbach. Result: t-test showed that spiritual score of respondent's intelligence after giving training there was a significant increase (p = 0,012). T-test analysis of respondents after the training there was an increase healthy life attitude score was significant (p = 0.047). Linear regression analysis showed a significant correlation (R = 861) increase in spiritual intelligence to increase healthy life attitude of employees of the plant. Conclusions: spiritual intelligence can improve significantly healthier life stance on factory labor in Yogyakarta.