DEVELOPMENT OF APTAMER BASED LATERAL FLOW IMMUNOCHROMATOGRAPHY STRIP TEST FOR HALAL AUTHENTICATION OF PHARMACEUTICAL PRODUCTS CONTAIN GELATIN
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The development of non-halal component detection technology is important because there are many cases of mixing non-halal ingredients with halal ingredients. The halal contaminant detection tool that can be applied in the field quickly, efficiently, portable and can be used for large amounts of samples such as the current strip test is still limited and must be imported from abroad. This study aims to develop rapid detection of halal contaminants in food and pharmaceutical products with apatamer-based lateral flow immunochromatography (single-strand oligonucleotides / ssDNA). The principle of making immunochromatographic test strips is to conjugate colloidal gold as a marker with aptamer. This conjugate is placed on the conjugate pad, one part of the immunochromatographic strip test system. Analysis of food/ pharmaceutical products with immunochromatographic test strips using samples made with gelatin, both references from bovine, porcine, vegetable gelatin and a mixture of gelatin from bovine-porcine and vegetable-porcine and also pharmaceutical products in the form of capsules on the market. The results of this research up to this stage have been obtained by the oligonucleotide sequence (ssDNA aptamer) which will be used to design the LFI test strip. One of the sequences is as follows: 5 '-GCCTAAAATCTCCCCTCCATGGATA- TGCCTAAATCTTCC-CTCAATGGTATGGCTAAATCTCCCCCCCCCGTAAGGCTAAA-TCTTCCTT-3'. Furthermore, aptamer candidates who will be used as biosensors will be purchased from suppliers in the modified position 5 'so that it can be applied in the LFI strip test system.