Hospitals are expected to provide service according to accreditation committee standard. One of the accreditation criteria is Infection and Prevention Control, in which hospital must minimalise infection risk from the provided facilities such as hospital food service. Analysing food handlers hygiene and sanitation practice in PKU Muhammadiyah Gamping Hospital's Food Service Unit. Research method is qualitative method, rand the design in a case study. Subjects of the research are 13 food handlers, one food service unit supervisor, one sanitation unit supervisor, one infection and prevention control unit supervisor. Here we conducted a questionnaire to measure knowledge, an observation check list to observe food handlers compliance, and in-depth interview for their perspective on hygiene and sanitation practice. Foodhandlers perspective in hygiene and sanitation practice, on kowledge wise 10 (76, 92%) foodhandlers have good knowledge on hygiene and sanitation. 13 (100%) foodhandlers have a good practice and compliance on practicing food safety and hygiene and sanitation practice. The Conclusion and Advice there are some adversities such as neglection to wear personal protective equipments and some facilities need to be upgraded.