OPTIMASI STERILISASI MENGGUNAKAN HIDROGEN PEROKSIDA (H2O2)PADA KULTUR IN VITRO EMBRIO KEPEL (Stelechocarpus burahol [Bl.] Hook. F. & Thomson)
Abstract
Kepel or which has a Latin name (Stelechocarpus burahol [Bl.] Hook. F. & Thomson) are native plants of Yogyakarta. Kepel plants are one of the rare plants and are not widely cultivated due to lack of economic attractiveness. The reason is that the method of multiplication is difficult to do. One method of propagation that can be done is by way of In vitro culture. The study was carried out by a single factor laboratory experiment method consisting of 6 treatments compiled with Completely Randomized Design (CRD). Each treatment was repeated 3 times with each test consisting of 3 samples to obtain 54 units. The treatment given is H2O2 concentration of 5%, 10%, 15% for 10 minutes and 15 minutes. Research results of kepel embryo explants sterilized using H2O2 solutions with a concentration of 5%, 10%, and 15% and immersion duration for 10 minutes and 15 minutes showed the percentage of explants live 100%, percentage contamination by 0%, and browning percentage of 22, 22%.