Introduction: The Urinary Tract Infection (UTI) is an infectious disease caused by bacterial growth and an infection in any part of urinary system such as urethra, bladder, ureters and kidneys. Antibiotic is a routine therapy for treating infectious diseases due to bacteria. One of the rational usage of antibiotics is the difference and variation of sensitivity pattern of bacteria that cause UTI. Objective: To determine the use of antibiotics in adult patients with UTI at inpatient installations of PKU Muhammadiyah Yogyakarta Hospital at the period of January-December 2017. Method: This study is a non-experimental study with descriptive observational research design and retrospective data collection. The sample of this study is inpatients diagnosed with urinary tract infection and recorded in PKU Muhammadiyah Yogyakarta Hospital Medical Record Installation period January-December 2017 included in the inclusion criteria. Results: The data taken came from 61 medical records. The single most widely used antibiotic was ceftriaxone in 25 cases (44,64%) and the most frequent combination antibiotic was ceftazidime + levofloxacin in 2 cases (40%). The appropriate use of antibiotics in 61 patients (100%), appropriate types in 61 patients (100%), appropriate duration of administration in 54 patients (88,52%), appropriate dose in 61 patients (100%), appropriate interval in 60 patients (98,36%) and appropriate route of administration in 61 patients (100%). The qualitative evaluation of antibiotic prescribing with the Gyssens method found 61 prescriptions (100%) in the IVA category (there are other more effective antibiotics)