Jamu is an traditional herbal medicine which is commonly used in traditional society to prevent and help relief many common symptoms of a disease. The rising of jamu usage in society have been misused by some jamu manufacturer by adding some synthetic chemical excipient (adulteration) such as paracetamol to increase the efficacy of the herbal. This research aimed to determine the appearance of paracetamol inside the rheumatic pain and arthritis gout jamu product in Yogyakarta city and the concentration of paracetamol. Samples were taken randomly from many jamu outlets widespread around Yogyakarta and the analysis was done in Laboratorium of Pharmacy Technology Universitas Muhammadiyah Yogykarta. The method of analysis used is TLCDensitometry. Qualitative analysis used TLC and the spot detected using UV light at 254 nm before calculating the Rf value. Quantitative analysis used densitometry by calculating area produced by the spot on TLC plate. The result of qualitative analysis showed that there are three of 14 samples of jamu containing Paracetamol drug. The three samples are sample number 3, 7 and 10. The result of quantitative analysis showed that each of the three contaminated samples containing 0,045 %, 0,350 % and 0,148 %.