THE EFFECTIVENESS OF INCORPORATION PROCESS OF PLATELET-RICH PLASMA IN GELATIN HYDROGEL BONE GRAFT BETWEEN IMPREGNATED AND DROP METHOD
MAHANANI, ERLINA SIH
ULZANAH, FANNISA AFRILYANA
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Tissue Engineering which involve three main component such as bone graft, platelet-rich plasma (PRP) and cells is expected to support in bone regeneration. Gelatin hidrogel bone graft is planted have a function as cell environment and PRP provide growth factor to support differentiation of cells. The success of tissue engineering is affected by number of PRP which is contained in bone graft. Purpose. The purpose of this study is to compere incorporation process between impregnated and drop method to gelatin hidrogel bone graft. Methods. This study was experimental research, Post Test Design that involve three donors for blood collection. PRP was prepared by taken 10ml of bloods for each donor. All of bloods were centrifugated twice using refrigerated centrifuge. The PRP was collected from the 2nd centrifugation by pipeting between buffy coat and red blood cell layer, then was incorporated into gelatin hydrogel bone graft. The effectiveness incorporation wascompared between impregnated and drop method. Results. Data were analyzed using independent sample t test. Result show the significant was 0,262 ( p>0,05 ) its mean there’s no significane different between impregnated and drop method. Conclusions. There’s no significant difference incorporation process between impregnated and drop methods. The number of platelet which incorporated in bone graft were effected by characteristic of bone graft such as structure, interface adherence, porosity and swelling ability. The good characteristic of bone graft could be obtain from synthesis and good fabrication technique.