Background: Cancer is one of the highest causes of death in the world. Colon cancer ranks number 3 with the highest incidence in Indonesia. Based on incidence, efforts should be made to prevent the spread of cancer as early as possible given the risks that can be caused. Cancer treatment using medical therapy requires high costs and has side effects. Soursop leaves have various contents of alkaloid, flafonoid, and acetogenin compounds for that research will be conducted on the effect of ethanol extract of soursop leaves (Annona mmuricata L.) in inhibiting WiDr colon cancer cells in vitro. Methods: This research method uses a quantitative experimental laboratory. The series of studies included extra ethanol cytotoxic tests of soursop leaves (Annona mmuricata L.) on colon WiDr cancer cells using MTT to assess cell viability and antimigration testing using the scratch wound healing assay method to assess the presentation of closure of WiDr colon cancer cell migration. Result: Cytotoxic test of ethanol extract of soursop leaves (Annona mmuricata L.) on colon WiDr cancer cells obtained IC50 values of 905.77 μg / mL and the cell antimigration test at 24 hours obtained the greatest migration inhibition, namely etanaol extract of soursop leaves (Annona mmuricata L .) at a dose of ½ IC50 or equal to 452.85 μg / mL. Conclusion: Ethanol extract of soursop leaves (Annona mmuricata L.) has an effect on inhibiting migration of WiDr colon cancer cells so that it can be used instead of chemotherapy which can prevent and inhibit the growth of cancer cells.