Background: Diabetes mellitus is one of the life-threatening disease. One alternative management of diabetes mellitus was fasting on Mondays and Thursdays. Objective: To determine the effect of fasting on Mondays and Thursdays to random blood glucose levels in people with type 2 diabetes mellitus. Methods: Quasi-Experimental pre-test and post-test with control group design. The research was conducted from April to May 2016 in Dukuh Kasihan. Respondents consisted of 15 people in experimental group were given intervention fasting on Mondays and Thursdays for 1 month and 15 people in control group who were not given the intervention by total sampling technique. Data were analyzed using Wilcoxon test and Independent T-Test with a significance level of p <0.05. Results: Mean of age and length of suffering diabetes was 57.20 years old and 4.53 years in experimental and 54.67 and 6.13 years in control. Eight people in experimental and 9 in control were taking metformin.. Fasting on Mondays and Thursdays have made random blood glucose levels lower in experimental group (p=0.05). There are differences after fasting on Mondays and Thursdays between experimental and control in which blood glucose on experimental are lower than control (p = 0.031). Conclusion: Fasting on Mondays and Thursdays has potential to decrease random blood glucose levels. Nurses may use fasting on Mondays and Thursdays as an optional intervention to decrease blood glucose. Next research can test the effect of fasting on Mondays and Thursdays to other variables such as uric acid and blood pressure by controlling confounding variables closely