Background: Hospital is one of the health care facilities to improve health status and human nutrition, if food from a Nutrition Installation in a hospital is contaminated with microorganisms, it can cause foodborne disease in the hospital. Foodborne disease is one of the supporting factors for nosocomial infections in hospitals. Objective: To analyze the effect of knowledge of Personal Protective Equipment (PPE) usage with the number of hand germs of nutritional installation officers of PKU Muhammadiyah Gamping Hospital, so as to reduce the incidence of foodborne disease PKU Muhammadiyah Gamping Hospital. Method: This is a study quantitative research, adopting observational analytic with the design cross sectional. The population and the sample are food nutrition installation PKU Muhammadiyah Gamping Hospital limestone are 13 people. Variable free this research level knowledge by variable bound numerical quantities germ figures and variable a bully contaminants when collecting sample. The method for collecting sample uses a questionnaire and hand swab. Results: The frequency of knowledge discharging PPE at the installation nutrition officers of PKU Muhammadiyah Gamping Hospital limestone most is 54% of the total respondents knowledgeable, 46% knowledgeable high. The germ figures hand installation nutrition officers have the germ hand 61,50% high category and as many as 38,50% low category. The germ highest is 3000 CFU/cm2 and the lowest 50 CFU/cm2. Conclusion: The level of knowledge using PPE nutrition installation officers are moderate category. The number of germ figures hand nutrition installation officers are high category. There is no influence between level of knowledge of using PPE with hands germ of the nutrition installation officers of PKU Muhammadiyah Gamping Hospital.