Aloe vera and chitosan have anti-bacterial property. Thus, they are potentially used for biomedical application such as wound dressing and wound healing. This research aims to study the morphology and tensile properties of AVG, chitosan, and PVA. There are four nanofiber membrane specimens used in this research: i.e. neat-PVA, AVG (2%)/PVA, AVG (2%)/CS (8%)/PVA, and AVG (2%)/CSNE (8%)/PVA. The nanofiber membranes were prepared by electrospinning method at hight DC voltage 18kV, TCD (Tip to Collector Distance) 16,5 cm, and needle diameter 0,6 mm. The viscosity of all spinning solutions before electrospinning process was measure with a viscometer. The morphology of the membranes was characterized by Scanning Electron Microscope (SEM) and tensile test of the membranes was conducted refer to ASTM D882. The results showed that the addition of AVG into PVA resulted in higher fiber diameter of the AVG (2%) /PVA membrane (194,3 nm) than that of PVA membrane (175, 6 nm). The fiber diameter of AVG (2%)/CS (8%)/PVA and AVG (2%)/CSNE (8%)/PVA are the 188,2 nm and 158,5 nm, respectively. The addition of AVG and chitosan increased the tensile properties of the membranes in which the highest tensile strength reached by AVG (2%)/CSNE (8%)/PVA membranes indicating that the addition of CSNE decreased the fiber diameter and significantly improved the tensile strength of the membrane, and included in the range of the native skin properties.