This study aims to describe the structure of chili supply chain relationships based on actors and their activities and to know the process of supply chain business of red chili in Panjatan District, Kulonprogo Regency, according to the chain business process relationship, distribution patterns, supporting actors, collaborative planing and research, collateral brand identity, risk aspects, and trust building. This research was conducted in Kulonprogo Regency which was chosen deliberately considering it has the highest level of red chili production in Special Region of Yogyakarta. Sampling in this study was carried out in several stages. The first stage was in Panjatan District, the second stage was in Garongan Village, and the third stage was in Sub-Village 1 and 3. Garongan Village was chosen for the reason that in 2018 it has the highest production of red chili in Panjatan District. Farmer respondents were determined using purposive sampling method. While the other respondents such as middlemen, Chairman of the Auction Market, Collector Traders, Bandar of PIKJ, Centheng of PIKJ, Retailers, and consumers were chosen using snowball sampling. The results showed that the structure of red chili supply chain relationship was created by 8 actors consisting of 3 chains, and had various activities such as planting, sorting, packaging, pricing, price quotations, information updates, purchasing, transporting, shipping, receive payments, sales, and cleanse or separate of red chili. The business process relationship consists of 3 cycle views and push or pull view, with all three chains in the same condition. There were 9 supporting actors in the red chili supply chain business process and various collaborative studies have been carried out by various agencies and academics. In addition, there were 6 risks faced by chain actors and there was no brand from the products they distributed. Cooperation that takes place along the supply chain was not formally bound by contract.