The purpose of this research was to determine the effect of structural assurance, perceived reputation, and design website toward trust of internet users’ in e-commerce systems. The sample used in this research is internet users’ at D.I.Y who have trade in e-commerce systems using purposive sampling method. The sample used was 68 people, the sample was obtained by distributing data directly and through Google Form directly to the respondent. The result of this research indicate that structural assurance and design website have significant effect on trust, while perceived reputation has no significant effect on trust of internet users’ in e-commerce systems. This means perceived reputation has not beenable to encourage internet users’ trust in e-commerce systems.