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dc.contributor.advisorARSITO, PUGUH NOVI
dc.contributor.authorMUTIARA, INDAH
dc.date.accessioned2016-09-28T03:24:16Z
dc.date.available2016-09-28T03:24:16Z
dc.date.issued2016-08-29
dc.identifier.urihttp://repository.umy.ac.id/handle/123456789/3181
dc.descriptionLada putih (Piper nigrum L.) mengandung Piperin. Piperin dapat menghambat pelepasan histamin dari sel mast dengan jalan menghambat jalur signal yang dimediasi oleh IgE. Piperin diduga memiliki aksi antagonisme terhadap reseptor asetilkolin. Tujuan penelitian untuk mengetahui pengaruh alkaloid lada (Piper nigrum Linn.) secara in vitro terhadap kontraksi otot polos ileum dan untuk mengetahui afinitas senyawa piperin terhadap reseptor asetilkolin secara in silico. Biji Piper nigrum Linn. di sokhletasi menggunakan pelarut etil asetat. Hasil ekstraksi diidentifikasi menggunakan KLT, uji titik lebur, FTIR dan spektrofotometri UV-Vis. Alkaloid lada Piper nigrum Linn. diuji in vitro dosis 1000 μM dan 5000 μM untuk melihat respon kontraksi dan relaksasi organ ileum marmut terisolasi. Uji in silico dilakukan untuk melihat skor Piper nigrum Linn. terhadap reseptor ACh menggunakan perangkat lunak AutoDock. Data hasil pengujian in vitro dianalisis menggunakan one way ANOVA dan LSD dengan taraf kepercayaan 95%. Hasil identifikasi pada uji KLT menunjukkan adanya bercak coklat muda sampai kuning (positif mengandung alkaloid). Titik lebur senyawa alkaloid Piper nigrum Linn. adalah 122-1320C (kurang murni) dan hasil uji spektrofotometri menunjukkan bahwa senyawa tersebut memiliki gugus fungsi C-H aromatik (3008,95), C=C asimetrik dan simetrik (1635,4), C=C aromatik (1581,63), -C-0- N- (1635,4), C-O (925,83), =C-O-C (1249,87) serta memiliki panjang gelombang maksimum 342,5 nm. Nilai pD2 Piper nigrum Linn. dosis 1000 μM dan 5000 μM adalah berturut-turut 3,89 dan 3,94. Nilai pD2 tersebut tidak beda signifikan (p<0,05) dalam menghambat kontraksi otot polos ileum. Alkaloid lada Piper nigrum Linn. mampu menghambat kontraksi ileum marmut yang diinduksi agonis asetilkolin dan memiliki skor docking pada reseptor ACh sebesar -6,6.en_US
dc.description.abstractWhite pepper (Piper nigrum L.) containing piperine. Piperine inhibits release of histamine from mast cells (inhibiting the signaling pathway mediated by IgE) and alleged to have action an antagonism of acetylcholine receptor. The aim of this research was to know the effect of alkaloids pepper (Piper nigrum Linn.) against ileum smooth muscle contraction with in vitro study and to determine the affinity of piperine against the acetylcholine receptor with in silico study. Seeds of Piper nigrum Linn. was extracted by soxhletation method using ethyl acetate as solvent. The results of extraction were identified by TLC, melting point test, FTIR and UV-Vis spectrophotometry. Alkaloids pepper Piper nigrum Linn. 1000 μM and 5000 μM were tested with in vitro study to see the response of contraction and relaxation of isolated guinea pig ileum organ and in silico study was be done to see the scores of Piper nigrum Linn. on ACh receptors using Autodock software. Data from in vitro study were analyzed using one-way ANOVA and LSD with confidence level at 95%. The results of the identification of the TLC test showed a brown to yellow spot (positive alkaloid). The melting point of alkaloid Piper nigrum Linn. was 122-1320C (less pure) and the result of spectrophotometric assay indicated that the compound has an aromatic CH functional group (3008.95), C = C asymmetric and symmetric (1635.4), C = C aromatic (1581.63), - C-0-N- (1635.4), CO (925.83), = COC (1249.87) and has a maximum wavelength at 342.5 nm. The value pD2 of Piper nigrum Linn. dosage 1000 μM and 5000 μM are respectively 3.89 and 3.94. The pD2 value did not differ significantly (p<0.05) in inhibiting contraction of ileum smooth muscle. An alkaloid pepper Piper nigrum Linn. can inhibit the contraction of guinea pig ileum which was induced by acetylcholine agonists and this alkaloid has a score of docking on ACh receptors at -6.6.en_US
dc.publisherFKIK UMYen_US
dc.subjectACh receptors, alkaloid pepper, ileum, in silico, piperine, Piper nigrum L,en_US
dc.titleUJI AKTIVITAS ANTAGONISME ISOLAT ALKALOID LADA (Piper nigrum Linn.) PADA RESEPTOR ASETILKOLIN OTOT POLOS ILEUM MARMUT TERISOLASI: STUDI IN VITRO DAN IN SILICOen_US
dc.typeThesis SKR 327en_US


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