UJI AKTIVITAS ANTAGONISME ISOLAT ALKALOID LADA (Piper nigrum Linn.) PADA RESEPTOR ASETILKOLIN OTOT POLOS ILEUM MARMUT TERISOLASI: STUDI IN VITRO DAN IN SILICO
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White pepper (Piper nigrum L.) containing piperine. Piperine inhibits release of histamine from mast cells (inhibiting the signaling pathway mediated by IgE) and alleged to have action an antagonism of acetylcholine receptor. The aim of this research was to know the effect of alkaloids pepper (Piper nigrum Linn.) against ileum smooth muscle contraction with in vitro study and to determine the affinity of piperine against the acetylcholine receptor with in silico study. Seeds of Piper nigrum Linn. was extracted by soxhletation method using ethyl acetate as solvent. The results of extraction were identified by TLC, melting point test, FTIR and UV-Vis spectrophotometry. Alkaloids pepper Piper nigrum Linn. 1000 μM and 5000 μM were tested with in vitro study to see the response of contraction and relaxation of isolated guinea pig ileum organ and in silico study was be done to see the scores of Piper nigrum Linn. on ACh receptors using Autodock software. Data from in vitro study were analyzed using one-way ANOVA and LSD with confidence level at 95%. The results of the identification of the TLC test showed a brown to yellow spot (positive alkaloid). The melting point of alkaloid Piper nigrum Linn. was 122-1320C (less pure) and the result of spectrophotometric assay indicated that the compound has an aromatic CH functional group (3008.95), C = C asymmetric and symmetric (1635.4), C = C aromatic (1581.63), - C-0-N- (1635.4), CO (925.83), = COC (1249.87) and has a maximum wavelength at 342.5 nm. The value pD2 of Piper nigrum Linn. dosage 1000 μM and 5000 μM are respectively 3.89 and 3.94. The pD2 value did not differ significantly (p<0.05) in inhibiting contraction of ileum smooth muscle. An alkaloid pepper Piper nigrum Linn. can inhibit the contraction of guinea pig ileum which was induced by acetylcholine agonists and this alkaloid has a score of docking on ACh receptors at -6.6.