Background: Estrogen is one of the hormones produced by the ovary. Estrogen plays an important role in the physical and non-physical aspects of females. Lack of estrogen can cause various changes in females. The reduction of estrogen is also a sign of menopause, where menopause is one of the conditions that will be experienced by elderly females. Most menopausal females experience menopausal symptoms, such as physical, psychological and social symptoms. Complaints of menopausal women in the oral cavity include gingivitis and burning mouth syndrome. Traumatic ulcer is one of the complaints in the oral cavity caused by trauma, it can be physical, mechanical and chemical trauma. To overcome complaints related to decreased estrogen, it can be increased using phytoestrogens. Phytoestrogens are compounds that have estrogen-like properties. Phytoestrogens are commonly found in legumes, one of which is soybeans. Soybeans contain flavonoids, one of the flavonoid compounds that are anti-inflammatory is isoflavones. Antiinflammation of isoflavones can play a role in wound healing. Epithelial thickness is one of the parameters seen from wound healing. Objective: To determine the effect of soybean flour extract on epithelial thickness on traumatic ulcer healing of Sprague-Dawley rats that have estrogen deficiency. Material and methods: This study was an experimental laboratory with 20 Sprague-Dawley rats divided into 5 groups, with 1 control group and 4 treatment groups. Wound healing was seen on day 1,3,5,7 by observing epithelial thickness using a microscope at 100x magnification. Results: The statistical test on day 1.5.7 showed a p value of <0.05 , this value showed there were significant differences between groups. The statistical test on day 3 shows a value of p> 0.05 this value showed there are significant differences between groups. Conclusion: This study conclude that there was an effect of soy tempeh flour extract on the healing of traumatic ulcers.