Background: Estrogen hormones work by binding to estrogen receptor-specific respectors (ERα and ERβ). Estrogen receptors are also found in the oral cavity as in the gingiva, so the hormone estrogen affects the health of the gingiva. Traumatic ulcers are local damage due to trauma characterized by loss of epithelial tissue. Phytoestrogens are one of the natural compounds found in plants such as soybeans that have a chemical structure similar to the body's estrogen and can bind to estrogen receptors. The purpose of this study was to determine the effect of giving soybean flour extract on traumatic ulcers of rats with estrogen deficiency, seen from the number of polymorphonuclear leukocytes (PMN). Method: This study was a laboratory experimental study using 20 female Sprague Dawley rats that were grouped into 5 groups and each group induced traumatic ulcers. Ovariectomy was performed in Groups 2, 3 and 4, with Group 3 being given soybean tempe flour extract orally. Group 4 was the ovariectomy and ulcer induction group without extract. The application of Kenalog to the ulcer was performed in Group 1 and Group 2. Group 5 was the group treated with ulcer induction without ovariectomy and without Kenalog application. The ELISA test was carried out to determine the concentration of estrogen in rat blood and the observation of the number of PMN cells in the ulcer was carried out using Hematoxylin eosin (HE) staining microscopically. Observation of the number of traumatic ulcer PMN cells was carried out on the first, third, fifth and seventh days after induction of traumatic ulcers. Results: There was an increase in estrogen concentration in the group by giving soybean flour extract and a decrease in estrogen concentration in the ovariectomy group without the administration of soybean tempe flour extract. The five groups carried out statistical tests using SPSS to find out whether there were differences in the number of PMN cells per group on the 7th day after ulcer induction. From the results of the test with SPSS there were no significant differences. Descriptively, the seventh day after ulcer induction was found in the number of PMN cells that increased in Group 4 and Group 1 when compared to the previous day. Decreasing number of PMN cells was found in Group 2, and 3. While Group 5 did not decrease compared to the fifth day after ulcer induction. Conclusion: The soybean tempe flour extract did not significantly influence the number of PMN cells in the traumatic ulcer of female Sprague Dawley rats with estrogen deficiency.