Background: DFU is a chronic wound infected by the bacteria. The initial stage of chronic wound formation is characterized by the appearance of aerobic, and then anaerobic bacteria. The study was to determine the effect of modern dressing combination of ozone bagging and honeying in reducing the number of anaerobic isolates of DFU. Method: This study uses a quasi-experimental method of pretest and post-test control group by accidental sampling. The respondents of this study were outpatients at the Kitamura Clinic. The subjects of this study consisted of 38 respondents with a control group of 19 respondents, and intervention of 19 respondents. Respondents were given a modern dressing combination of ozone bagging and honey for 4 treatments, then anaerobic germ examination, and wound measurement were performed. Result: The results of the study anaerobic bacteria in the intervention group were 301 CFU (pretest) and 36 CFU (posttest), in the control group 303 CFU (pretest) and 102 CFU (posttest), (p <0.05). Wound scores in the intervention group 8.42 (pretest) and 6.79 (posttest), while the control groups 8.79 (pretest) and 7.95 (posttest) (p <0.05). Conclusion: The conclusion of this study is wound care using a modern dressings combination of ozone bagging and trigona honey can reduce the number of anaerobic bacteria isolates and accelerate the process of wound healing DFU. There is a difference between modern wound care dressing combined with ozone bagging and trigona honey in reducing the number of anaerobic bacteria isolates and wound healing process, and there is no relationship between the number of anaerobic bacteria isolates with the wound healing process DFU.