This study aims to analyze the Effect of Entrepreneurship Education on Entrepreneurial Interest with Self-Efficacy as an Intervening Variable in Students of the Faculty of Economics and Business, University of Muhammadiyah Yogyakarta. The subjects in this study were students of the Faculty of Economics and Business, University of Muhammadiyah Yogyakarta, 2016, who had taken entrepreneurship education courses. In this study the number of respondents was 268 respondents who were determined using the Slovin formula. In addition, in this study using a purposive sampling method, namely determining respondents with criteria - criteria. This research uses Structural Equation Modeling (SEM) which is run through the AMOS program as an analysis tool. Based on the analysis that has been done, the results show that entrepreneurship education has a significant positive effect on entrepreneurial interest, entrepreneurship education has a significant positive effect on self-efficacy, self-efficacy has a significant positive effect on entrepreneurial interest, and self-efficacy mediates positively and significantly between entrepreneurship education. towards entrepreneurial interest.