This study aims to determine the effect of performance appraisal, participation, compensation, organizational culture, work stress, internal locus of control and external locus of control on performance. This study uses performance as the dependent variable and appraisal performance, participation, compensation, organizational culture, work stress, internal locus of control and external locus of control as independent variables. The sample used was employees and lecturers of private universities in Yogyakarta. This research uses incidental method. The data used are primary data. Based on the questionnaire distribution process, 150 respondents were obtained. Data processing using SPSS 16.0 and the analytical method used is multiple regression analysis. The results of this study indicate that performance appraisal, participation, compensation and organizational culture have a positive effect on performance. Job stress has a negative effect on performance. Internal locus of control and external locus of control do not affect performance.