Abstract The incidence of tuberculosis in Yogyakarta is 221 cases, 60.18% male and 39.82% women. These conditions are influenced by the lack of adherence to taking medication and carrying capacity of the family. Another problems are the lack of optimal role of health cadres in health promotion and tuberculosis in the community. Cadres are the key to the success of the tuberculosis control program, because cadres have a role as family counselors, finding suspects early, referring to residents and supervisors to swallow drugs for tuberculosis patient directly. The aim of the study was to analyze the influence of tuberculosis prevention counseling on health cadres knowledge and attitudes. The research method is experimental, pre-test and post-test group design.Counseling uses a kit containing posters, videos and leaflets. The kit contains material about the importance of family, adherence to taking medication and nutrition in dealing with tuberculosis. Before and after the intervention, the cadres knowledge and attitudes were measured against tuberculosis prevention efforts with a standardized questionnaire. Data were analyzed to compare with the non-parametric Wilcoxon method test. The number of subjects who fulfilled the inclusion and exclusion criteria were 62 health cadre subjects. The characteristics of the study subjects showed that the highest level of education was high school 36 subjects (58.1%), junior high school 12 subjects (19.4%), elementary school 5 subjects (8.1%) and undergraduate 4 subjects (6.5%). The results showed that there was a significant relationship between counseling using the kit above with knowledge and attitudes towards tuberculosis prevention efforts in the Bantul area of Yogyakarta (p = 0,000). A good level of knowledge is expected to increase the prevention of tuberculosis transmission. In addition, improving the quality of cadres is expected to be an effort to increase knowledge in the community through the provision of communication, information and education by healthy cadres, so that these efforts will reduce the prevalence and incidence of tuberculosis. The conclusion of the study is a significant increase the level of knowledge and attitudes of health cadres in an effort to prevent tuberculosis cases after counseling with poster kits, videos and leaflets about the importance of family, adherence to taking medication and nutrition in dealing with tuberculosis.