Background: Chronic renal failure is a progressive chronic disease that needs kidney therapies, one of them is hemodialysis. In this case, patient compliance related to the frequency of hemodialysis can affect the success of therapy which is interpreted through iron status. This study discusses the relation in hemodialysis frequency towards iron status of chronic renal failure patients who experience hemodialysis. Method: This research used observational analytic method through a cross sectional analysis of trials. Number of samples in this research were 50 patient medical records. The independent variable of this research is the frequency of hemodialysis and the variable released is the iron status level. After the data was obtained from each variable, then it was analyzed by using the Spearman correlation test. Result: Research results obtained from chronic renal failure patients with Fe level increased by 2%, normal by 13%, and decreased by 72%. Whereas the TIBC level increased by 14%, normal 38%, and decreased by 48%. Furthermore, the SAT level increased by 2%, normal 36%, and decreased by 62%. The results of the analysis from the correlation test obtained significance figures p = 0.321 for Fe, p = 0.251 for TIBC, p = 0.018 for SAT, and p = 0,087 for Hb. There is no significant connection in Fe, TIBC, and Hb, while in SAT obtained a significant relation with the coefficient r = 0.034 (weak resistance). Conclusion: Based on these results, it can be concluded that there is no significant relationship between the frequency of hemodialysis on Fe, TIBC, and Hb and there is a significant relationship between the frequency of hemodialysis on SAT which shows a significant number p = 0.018 and a comparison coefficient r = 0.334.