Background: In Indonesia, the incidence of medical error is quite high as evidenced by the existence of hospital incident reports, where in 2010 there were 75% of unexpected events and in 2011 as many as 60% of cases of surgical cases or patients with surgery. The hospital should be able to carry out management based on customer oriented and patient safety by implementing a safety attitude culture Objective: to analyse determine the safety attitude culture evaluation of nurses in surgery rooms of PKU Muhammadiyah Gamping. Research Methods: This research was a research using a mixed methods research approach; namely a quantitative method and qualitative methods. The population in this study was nurses in surgery room with a total sampling technique of 20 people. Quantitative data analysis used descriptive analysis; while qualitative analysis was performed by data reduction, data presentation, and drawing conclusions/verification. Results: The results shows that overall evaluation of safety culture attitude of nurses in the surgery room summed up in the high category (75,0 %). Meanwhile based on the safety attitude culture component, namely safety climate is in the high category (85,0%), team working climate is in the high category (90,0%), stress recognition is in the high category (65,0%), management perceptions is in the moderate category ( 75,0%), working conditions is in the high category (60,0%), job satisfaction is in the high category (90,0%). Conclusion: High safety attitude culture of nurses will improve quality of patient care, especially patients who are in the surgery room. This can prevent the occurrence of nurses' medical errors in implementing nursing care to hospital patients. The higher safety attitude culture is expected to improve good performance for nurses and improve patient safety.