Dengue is a disease related to the environment. This study aims to identify the environmental conditions associated with dengue. The independent variables were elevation, land cover and climate. This ecological study was conducted on five sub-districts selected based on the trend of the incidence during 2008-2013. Land cover and elevation data obtained using GIS software from Quickbird imagery and digital maps respectively. Climate data obtained from Meteorology Climatology and Geophysics Agency of Yogyakarta Special Region. There were 1,150 dengue cases from 2008-2013 obtained from Sleman District Health Office. The Average Nearest Neighbour Test shows the spatial patterns of dengue in Sleman are clusters in all sub-districts (Z score <-2.58). Spearman's Rho Test showed there was a negative correlation between land cover by the incidence of DHF (p = 0.000; r = -0.837). Pearson Test showed there was a negative correlation between dengue and elevation (p = 0.000; r = -0.127). Multiple Regression Test shows the effect of humidity (p = 0.000) in high endemic areas, precipitation (p = 0.002) in moderate endemic area with a contribution of 13.5% - 27.4%. Temperature has no effect in all districts (p> 0.05). It concluded that dengue incidence in Sleman are cluster. The elevated the region, the lower the incidence. The wider building area, the lower the incidence. Climatic factors that influence are humidity and precipitation, not temperature.