The patients with type 2 Diabetes Mellitus who did not control their total cholesterol level properly ran the risk of variouscomplications. Diet plan was important to prevent complications ofthe patients with Type 2 Diabetes Mellitus caused by hypercholesterolemia. Fasting on Mondays and Thursdays was appropriate with Sunnah of Prophet Muhammad SAW and potential for reducingthe total cholesterol level of the patients with Type 2 Diabetes Mellitus. Research Objective: This study aimed to know the influence of fasting on Mondays and Thursdays towards the total cholesterol levelof the patients with Type 2 Diabetes Mellitus. Methodology: This study used Quasy-Experimental with pre-test and post-test control group design. This study was conducted on January-May 2016 in Kasihan, Bantul, Yogyakarta. The respondents consisted of 15 people in experimental group who were given intervention fasting every Monday and Thursday for one month with total sampling technique and 15 people in control group with standard treatment. The data was analyzed using Wilcoxon test and Independent T-Test with significance p<0.05. Research Results: The average of cholesterol level after intervention was 189.87±21.52 in the experimental group and 223.33±45.77 in the control group. There were significant differences in the total cholesterol level between experimental and control groups before and after having fasting on Mondays and Thursdays and it showed that the total cholesterol level of experimental group was lower than control group (p=0.033). Conclusion: Fasting on Mondays and Thursdayseffectively reducedthe total cholesterol level of the patients with Type 2 Diabetes Mellitus. Nurses could suggest the Diabetes Mellitus patients having fasting on Mondays and Thursdays with guidance ahead. The future study was suggested to find out the influence of fasting on Mondays and Thursdays towards control parameter of Diabetes Mellitus such as HbA1c, 2-hour postprandial blood glucose, and fasting blood glucose