This research aims to analyze the factors influence knowledge sharing in the implementation of e-learning in Universitas Muhammadiyah Yogyakarta. The subject in this study is that lectures in Universitas Muhammadiyah Yogyakarta. Sampling using non-probability sampling with purposive sampling type, in a way distributing questionnaires of 115 respondents. The analytical method used is multiple linear regression analysis and using validity, reliability test, classic assumption test, F test and t test. The results of this study indicate, first, that the employee attitude, motivation to share, communication and technology simultaneously significant effect on knowledge sharing with a significance of 0.000 < α 0.005. Second, the employee attitude have a negative impact and no significant regression coefficient evidenced by the value of the employee attitude is - 0.164. Another variable has a positive impact and significant regression coefficient evidenced by the value of the motivation to share is 0,482, regression coefficient value of the communication is 0.191 and regression coefficient of technology is 0.220. Analysis of the coefficient of determination shows the number of 0,403 equal to 40,3%, which means the employee attitude, motivation to share, communication and technology affect knowledge sharing by 40,3%, while the remaining 59,7% likely influenced by other factors not examined in this study.