This study aims to analyze the Influence of Entrepreneurship Education on Interest in Entrepreneurship with Self-Efficacy and Entrepreneurship Motivation as an intervening variable on Management students at universitas Muhammadiyah Yogyakarta. The subject in this study was students of Management, Universitas Muhammadiyah Yogyakarta force in 2013 and 2014 are already taking courses in Kewirausahaan, Perencanaan Bisnis dan Pengembangan Bisnis. In this study, the number of respondents were 275 respondents who are determined using the formula Slovin. Additionally, in this study using purposive sampling method to determine the respondents with the criteria. This study uses Structural Equation Modeling (SEM) that run through AMOS’s program as a tool of analysis. Based on the analysis that have been made the result are entrepreneurship education no significant effect on self-efficacy, entrepreneurship education positive significant effect on the entrepreneurship motivation, entrepreneurship education positive significant effect on the interest in entrepreneurship, self-efficacy positive significant effect on the interest in entrepreneurship and entrepreneurship motivation no significant effect on the interest in entrepreneurship.