Herbal Medicine is one uniqueness medicinal approach, basic way of thinking of traditional medical is human healthy by means of balance condition of human bodies. Body’s human balance should be approach systemic, holistic, and material, as well as immaterial domain. One case of complicated system in human is pregnancy, pregnant women are one of higher risk population for Salmonella typhii infection. Typhoid fever during pregnancy can cause decreasing immune system and imparity of the hormonal system. Indonesia have a lot of herbal medicine, there are Elephantopus scaber (Tapak Liman) and Sauropus androgynus (Katuk). Both can use as a herbal supplement for pregnant women because they have functioned as an anti-in ammatory effect, antimicrobial and stimulate reproduction hormone. The aim of this study was to investigate the ef cacy of E. scaber and S. androgynus as a food supplement for modulating immune and hormonal system in Pregnant mice with Salmonella typhii infection. Pregnant mice were orally infected with S. typhii, at a concentration 107 CFU/mL. Extract of E. scaber and S. androgynus were administered orally using formulation 100:0% (T3), 75:25% (T4), 50:50% (T5), 25:75% (T6), and 0:100% (T7), with initial doses of 200 mg/kg body weight (BW) E. scaber and 150 mg/kg BW S. androgynus. Lymphocyte cell was isolated from bone marrow, spleen, and lymph node. Pregnant BALB/c mice serum was isolated from the orbital vein. FACS analysis was done to measure the relative number of B220+ cell and CD4+IL2+ T cell. The hormonal level was analysis using ELISA assay. The data were analyzed using one-way ANOVA, with signi cance level p<0.05, followed by a Tukey test. The statistical analysis software used SPSS version 16.0. The result shows that the combination leaves extract of E. scaber and S. androgynus have ef cacy to modulated immune system such as B220+ in bone marrow and also lymph node, IL2, and CD4 T cell. Beside, both leaves extract are also modulating hormonal system like prolactin hormone in pregnant mice with S. typhi infection.