Background: In these past few years, automotive industrials are developed rapidly in Indonesia, which also given an impact on the employment of the workers. In the other hand, automotive industrials produce many pollutans such as benzene, lead, and carbon monoxide that can affect healthiness (of the human body) especially on the level of hemoglobin. Hemoglobin is the main compotition of blood contains a protein that compose the red blood cell. It transports most of the oxygen (𝑂2), fractions of carbon dioxide (𝐶𝑂2), and maintain the normal pH. Automotive workers (mechanic workers) are having higher risk on the decreased level of hemoglobin hence the fact that they were exposed by more pollutans than the workers who work inside the building such as cleaning service. This research is needed to perceive the difference of the hemoglobin level between workers whos exposed to the pollutans and workers whos not exposed to pollutans. Purpose: To to perceive the difference of the hemoglobin level between workers whos exposed to the pollutans and workers whos not exposed to pollutans. Methods: This research is an analytic observational study with cross sectional design. This research was done by collected blood samples of workers whos exposed to pollutans (mechanic woorkers) at Bantul and workers whos not exposed to pollutans at Universitas Muhammadiyah Yogyakarta in the range of February until May in 2017. Hemoglobin levels were examined from the blood sample in Balai Laboratorium Yogyakarta. Total samples is 40 samples consisted of 20 workers whos exposed to pollutans and 20 workers whos exposed to pollutans. Data was analyzed by Independent Samples Test. Results and Discussion: The average value of hemoglobin level of workers whos exposed to pollutans is 15.06 gr/dl and workers whos not exposed to pollutans is 15.74 gr/dl. So, it conclude that average value of hemoglobin level of workers whos exposed to pollutans is lower than workers whos not exposed to pollutans. There is a meaningful difference of hemoglobin level between workers whos exposed to pollutans and workers whos not exposed to pollutans. It can be proven from the result of Independent Samples Test which showed p < 0,05 (p = 0,021). Conclusion: This research concluded that there is a difference of hemoglobin level between workers whos exposed to pollutans and workers whos not exposed to pollutans.