This study aims to analyze the influence of work family conflict, the role of ambiguity, job stress and employee performance at PKU Muhammadiyah Temanggung hospital. The data used in this study was compiled by questionnaires and implemented in 70 female nurses at PKU Muhammadiyah Temanggung hospital, who are married and have family. Analysis techniques in this study include, validity test, classical assumption test, and path analysis. The results of this study indicate the existence of work family conflicthas positive and significant on job stress, role ambiguity has positive and significant on job stress, negative and significant job stress on the performance of employees, work family conflict negative and significant on the performance of employees, the role of ambiguity have a negative and significant effect on the performance of employees. In addition, job stress has a negative effect in the relationship between work family conflict and performance of employees in the relationship between role ambiguity and employee performance.