Universal precautions are the action of infection control conduted by health care practitioner to reduce the risk of infection spreading and based on the principle that blood and body fluid have the potency to transmit disease, not only from the patient, but also from the healthcare practitioner. Healthcare practitioners in public health care are obliged to preserve the safety of themselves, the ptients, and they need to have good knowledge. Intervention in form of education is necessary to improve the knowledge of the helathcare practitioner as an effort to prevent infection and self safety of healthcare practitioner. Method: This research is a quantitative research using experimental quasy non randomized research design with one grup pretest-posttest design with controlgroup. Sampling technique using probability sampling with purposive sampling. The are 30 healthcare practitioner as samples. Education is given for 30 minutes. Instruments of this research is a questionnaire with the content of education materials. Result: This study shows the calculation of P-value= 0.008 < 0.05 indicates that there is a significant increase of knowledge of healthcare practitioner after given the intervention in form of education about the use of syringe toward the knowledge of healthcare practitioner in Gedongtengen and Kotagede I Non Hospitalization Health Care. Conclusion and suggestion: education about the use of syringe has the effect toward the knowledge of healthcare practitioner in Gedongtengen and Kotagede I Non Hospitalization Health Care. There is the necessity to keep on sustainably providing education as the effort to prevent infection and for self safety during service.